Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR

Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR

Blog Article

Summary: Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, Shoe Horns yet the structural basis underlying the catalytic performance is challenging to probe.Here, we present an effective protocol to determine the orientation and dynamics of enzymes in MOFs/COFs using site-directed spin labeling and electron paramagnetic resonance spectroscopy.The protocol is demonstrated using lysozyme and can be generalized to other enzymes.For complete information on the Weight Plate Pin generation and use of this protocol, please refer to Pan et al.(2021a).